Expression of Pinellia ternata leaf agglutinin under rolC promoter confers resistance against a phytophagous sap sucking aphid, Myzus persicae
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چکیده
منابع مشابه
Myzus persicae Sulzer aphid contains oxylipins that
Oxylipins are oxygenated fatty acids and their derivatives that play key roles in response to stress. They are widespread in many organisms including mammals or plants but are unknown in arthropods. In this study, we identified oxylipins in Myzys persicae Sulzer aphids reared on Vicia faba L. plants. Further experiments based on: (i) Radiolabelled substrate incubation with aphid extracts, and (...
متن کاملPinellia ternata agglutinin produced in Bombyx mori cells exhibits bioactivity.
Pinellia ternata agglutinin (PTA) is highly homologous to many other monocot mannose-binding lectins which reportedly possess antitumor activities. Its production in silkworm cells has great application potential because the baculovirus expression system can produce post-translationally modified proteins at low cost. In the current study, the pta gene was cloned and expressed in silkworm cells,...
متن کاملInsecticidal effect of recombinant endophytic bacterium containing Pinellia ternata agglutinin against white backed planthopper, Sogatella furcifera
White backed planthopper (WBPH; Sogota furcifera Horvath) has become the major threat to rice crops throughout Asia, damaging plants both through its feeding behavior and by acting as a virus vector. Here, we developed a novel method for biologically controlling WBPH by using endophytic bacterium to express anti-pest plant lectins. Strain SJ-10 of an endophytic bacterium, characterized as Enter...
متن کاملExpression of Pinellia pedatisecta Lectin Gene in Transgenic Wheat Enhances Resistance to Wheat Aphids.
Wheat aphids are major pests during the seed filling stage of wheat. Plant lectins are toxic to sap-sucking pests such as wheat aphids. In this study, Pinellia pedatisecta agglutinin (ppa), a gene encoding mannose binding lectin, was cloned, and it shared 92.69% nucleotide similarity and 94% amino acid similarity with Pinellia ternata agglutinin (pta). The ppa gene, driven by the constitutive a...
متن کاملInsecticide Resistance Mechanisms in the Green Peach Aphid Myzus persicae (Hemiptera: Aphididae) I: A Transcriptomic Survey
BACKGROUND Insecticide resistance is one of the best examples of rapid micro-evolution found in nature. Since the development of the first synthetic insecticide in 1939, humans have invested considerable effort to stay ahead of resistance phenotypes that repeatedly develop in insects. Aphids are a group of insects that have become global pests in agriculture and frequently exhibit insecticide r...
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ژورنال
عنوان ژورنال: Electronic Journal of Biotechnology
سال: 2020
ISSN: 0717-3458
DOI: 10.1016/j.ejbt.2020.07.004